Human HDAC10 ELISA Kit
Cat no : KE00931
Synonyms
EC:3.5.1.48, HD 10, HD10, HDAC 10, Polyamine deacetylase HDAC10
Validation Data Gallery
Product Information
KE00931 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human HDAC10 ELISA kit is to be used to detect and quantify protein levels of endogenous human HDAC10. The assay recognizes human HDAC10. An antibody specific for human HDAC10 has been pre-coated onto the microwells. The human HDAC10 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human HDAC10 is added to detect the captured human HDAC10 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human HDAC10 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.55 ng/mL |
| Range | 1.25-80 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 83933 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 98% | 92%-106% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 113.31 | 4.53 | 3.99 |
| 2 | 8 | 30.09 | 0.86 | 2.85 |
| 3 | 8 | 7.81 | 0.35 | 4.52 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 119.46 | 10.07 | 8.43 |
| 2 | 16 | 31.15 | 1.76 | 5.65 |
| 3 | 16 | 7.99 | 0.54 | 6.81 |
Background Information
Histone deacetylase 10 (HDAC10) as a class II histone deacetylase, consists of 669 amino acid residues. This residue contains an N-terminal active deacetylase domain and a C-terminal leucine-rich domain (LRD) that is catalytically inactive. HDAC10 can participate in the regulation of tumorigenesis and development of various tumors.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:3.5.1.48, HD 10, HD10, HDAC 10, Polyamine deacetylase HDAC10 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.