Human HIRA ELISA Kit
Cat no : KE00717
Synonyms
HIR, HIRA/HIR, Protein HIRA
Validation Data Gallery
Product Information
KE00717 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human HIRA ELISA kit is to be used to detect and quantify protein levels of endogenous human HIRA. The assay recognizes human HIRA. An antibody specific for human HIRA has been pre-coated onto the microwells. The human HIRA protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human HIRA is added to detect the captured human HIRA protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human HIRA ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7290 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 91% | 89%-92% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 9.98 | 0.76 | 7.60 |
| 2 | 8 | 2.31 | 0.18 | 7.79 |
| 3 | 8 | 0.79 | 0.06 | 7.91 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 10.07 | 0.95 | 9.40 |
| 2 | 16 | 2.42 | 0.23 | 9.53 |
| 3 | 16 | 0.78 | 0.05 | 6.22 |
Background Information
HIRA (Histone Regulator A) is a key histone chaperone involved in the deposition of the histone variant H3.3 into chromatin. The histone chaperone HIRA complex, composed of HIRA, Cabin1 and UBN1, is an evolutionarily conserved histone chaperone, which has been widely reported as a critical chromatin regulator in senescent cells. Moreover, other histone chaperones assist HIRA in performing multiple activities: 1. DNA damage repair, 2. H3.3 enrichment regulation, 3. myogenic progression, etc.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | HIR, HIRA/HIR, Protein HIRA |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.