Human HNF4A ELISA Kit
Cat no : KE00634
Synonyms
Hepatocyte nuclear factor 4-alpha, HNF 4 alpha, HNF4, HNF4a7, HNF4a8
Validation Data Gallery
Product Information
KE00634 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human HNF4A ELISA kit is to be used to detect and quantify protein levels of endogenous human HNF4A. The assay recognizes human HNF4A. An antibody specific for human HNF4A has been pre-coated onto the microwells. The human HNF4A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human HNF4A is added to detect the captured human HNF4A protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human HNF4A ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.06 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3172 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 81% | 78%-83% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 21.72 | 0.84 | 3.86 |
| 2 | 8 | 5.13 | 0.15 | 2.96 |
| 3 | 8 | 2.58 | 0.10 | 3.78 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 20.07 | 1.88 | 9.35 |
| 2 | 16 | 4.84 | 0.37 | 7.65 |
| 3 | 16 | 2.46 | 0.15 | 5.91 |
Background Information
HNF4A (Hepatocyte nuclear factor 4 α) is highly conserved between humans and mice. Using conditional knockouts, epithelial HNF4A is indispensable for protecting against acute chemical-induced colitis and spontaneous colonic inflammation in aged animals. Moreover, reduced expression of HNF4A has been reported in liver fibrosis, and viral vector-mediated HNF4A restoration has previously been demonstrated to reverse features of chronic liver injury. Therefore, HNF4A has been suggested as a therapeutic target for treating liver fibrosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Hepatocyte nuclear factor 4-alpha, HNF 4 alpha, HNF4, HNF4a7, HNF4a8 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.