Human HNRNPA2B1 ELISA Kit
Cat no : KE00269
Synonyms
HNRNPA2B1,FLJ22720,hnRNP A2/B1,HNRNPA2,HNRNPB1
Validation Data Gallery
Product Information
KE00269 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HNRNPA2B1 ELISA kit is to be used to detect and quantify protein levels of endogenous HNRNPA2B1. The assay recognizes human HNRNPA2B1. An antibody specific for HNRNPA2B1 has been pre-coated onto the microwells. The HNRNPA2B1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for HNRNPA2B1 is added to detect the captured HNRNPA2B1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human HNRNPA2B1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 5.0 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3181 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 91% | 84%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,025.4 | 29.0 | 2.8 |
| 2 | 20 | 245.9 | 5.5 | 2.2 |
| 3 | 20 | 55.2 | 2.0 | 3.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,042.1 | 36.1 | 3.5 |
| 2 | 24 | 244.8 | 7.5 | 3.1 |
| 3 | 24 | 57.0 | 5.5 | 9.7 |
Background Information
HNRNPA2B1 is one of heterogenous nuclear ribonucleoproteins (HnRNPs), which regulate the splicing and transportation of mRNA. It has a role in packaging nascent mRNA, alternative splicing, cytoplasmic RNA trafficking, translation and stabilization. Besides it may involve in telomere maintenance, cell proliferation and differentiation, and glucose transport.HNRNPA2B1 is widely expressed in various tissues, and it's reported that it is highly expressed in many tumors. HNRNPA2B1 promotes tumor cell growth by activating the STAT3 pathway, regulating apoptosis and autophagy. HNRNPA2B1 is elevated in multiple myeloma patients and negatively correlated with favorable prognosis. It is found to activate cyclooxygenase-2 and promote tumor growth in human lung cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | HNRNPA2B1,FLJ22720,hnRNP A2/B1,HNRNPA2,HNRNPB1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.