Human HSP47/SERPINH1 ELISA Kit
Cat no : KE00365
Synonyms
HSP47,SERPINH1,47 kDa heat shock protein,Arsenic-t
Validation Data Gallery
Product Information
KE00365 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human HSP47/SERPINH1 ELISA kit is to be used to detect and quantify protein levels of endogenous human HSP47/SERPINH1. The assay recognizes human HSP47/SERPINH1. An antibody specific for human HSP47/SERPINH1 has been pre-coated onto the microwells. The human HSP47/SERPINH1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human HSP47/SERPINH1 is added to detect the captured human HSP47/SERPINH1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human HSP47/SERPINH1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.47-30 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 871 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 109% | 104%-118% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 13.78 | 1.10 | 8.00 |
| 2 | 20 | 3.36 | 0.29 | 8.57 |
| 3 | 20 | 0.79 | 0.06 | 7.95 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 15.77 | 1.08 | 6.86 |
| 2 | 24 | 3.85 | 0.28 | 7.39 |
| 3 | 24 | 0.96 | 0.09 | 9.59 |
Background Information
HSP47 is also named as Serpin H1, colligen, CBP1, CBP2, HSP47, SERPINH2 and belongs to the serpin family. This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum. It binds specifically to collagen and can be involved as a chaperone in the biosynthetic pathway of collagen. The Serpin Family H Member 1 (SERPINH1) gene encodes a protein called HSP47, which is required for the correct folding and secretion of collagen. SERPINH1 has been implicated in several collagen-related diseases, such as, brittle bone disease, keloid scars, and lung fibrosis. SERPINH1 also correlates with the grade of glioma, and regulates proliferation, migration and invasion of glioma cell lines. SERPINH1 regulates the Wnt/β-catenin signaling pathway and modulates gastric cancer (GC) progression SERPINH1 is aberrantly expressed in cervical cancer, breast cancer, glioblastoma and colorectal cancer. SERPINH1 promotes invasion and metastasis of breast cancer cells by regulating the expression of several extracellular matrix (ECM) proteins .
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | HSP47,SERPINH1,47 kDa heat shock protein,Arsenic-t |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.