Human Haptoglobin ELISA Kit
Cat no : KE00148
Synonyms
BP, HAPT, haptoglobin, HP, HP2 ALPHA 2, HPA1S
Validation Data Gallery
Product Information
KE00148 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Haptoglobin ELISA kit is to be used to detect and quantify protein levels of endogenous Haptoglobin. The assay recognizes human Haptoglobin. An antibody specific for Haptoglobin has been pre-coated onto the microwells. The Haptoglobin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Haptoglobin is added to detect the captured human Haptoglobin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Haptoglobin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine, Saliva |
| Assay type | Sandwich |
| Sensitivity | 3.0 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3240 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 101% | 72%-120% |
| Cell culture supernatants | 87% | 72%-95% |
| Urine | 96% | 73%-113% |
| Saliva | 99% | 85%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,795.3 | 81.6 | 4.5 |
| 2 | 20 | 460.1 | 25.4 | 5.5 |
| 3 | 20 | 109.1 | 10.8 | 9.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,796.6 | 99.5 | 5.5 |
| 2 | 24 | 425.7 | 16.7 | 3.9 |
| 3 | 24 | 101.0 | 9.9 | 9.8 |
Background Information
Haptoglobin(HP), also named as zonulin, is a plasma glycoprotein and belongs to the peptidase S1 family. As a kind of acute-phase response protein, serum Hp is mainly synthesized by hepatocytes and is widely distributed in the circulation in humans. The main physiological function of serum Hp is binding free haemoglobin released from red blood cell lysis and protecting tissues and vessels from oxidative damage. The level of Hp is a useful biomarker for some diseases diagnosis, such as Hepatocellular carcinoma, Pancreatic cancer, Type 2 diabetes mellitus, Diabetes kidney disease and so on.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BP, HAPT, haptoglobin, HP, HP2 ALPHA 2, HPA1S |
Publications
| Species | Sample Type | Title |
|---|---|---|
Stem Cell Res Ther Haptoglobin is an early indicator of survival after radiation-induced severe injury and bone marrow transplantation in mice | ||
Ann N Y Acad Sci Identification of maternal serum biomarkers for prenatal diagnosis of nonsyndromic orofacial clefts |