Human Haptoglobin ELISA Kit

Sensitivity

3.0 pg/mL

Range

62.5-4000 pg/mL

Reactivity

Human

Cat no : KE00148

Synonyms

BP, HAPT, haptoglobin, HP, HP2 ALPHA 2, HPA1S



Product Information

KE00148 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Haptoglobin ELISA kit is to be used to detect and quantify protein levels of endogenous Haptoglobin. The assay recognizes human Haptoglobin. An antibody specific for Haptoglobin has been pre-coated onto the microwells. The Haptoglobin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Haptoglobin is added to detect the captured human Haptoglobin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human Haptoglobin ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Urine, Saliva
Assay type Sandwich
Sensitivity 3.0 pg/mL
Range 62.5-4000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human serum101%72%-120%
Cell culture supernatants87%72%-95%
Urine96%73%-113%
Saliva99%85%-112%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1201,795.381.64.5
220460.125.45.5
320109.110.89.9

InterAssay

Samplenmean ( pg/mL)SDCV%
1241,796.699.55.5
224425.716.73.9
324101.09.99.8

Background Information

Haptoglobin(HP), also named as zonulin, is a plasma glycoprotein and belongs to the peptidase S1 family. As a kind of acute-phase response protein, serum Hp is mainly synthesized by hepatocytes and is widely distributed in the circulation in humans. The main physiological function of serum Hp is binding free haemoglobin released from red blood cell lysis and protecting tissues and vessels from oxidative damage. The level of Hp is a useful biomarker for some diseases diagnosis, such as Hepatocellular carcinoma, Pancreatic cancer, Type 2 diabetes mellitus, Diabetes kidney disease and so on.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms BP, HAPT, haptoglobin, HP, HP2 ALPHA 2, HPA1S