Human Haptoglobin ELISA Kit
Cat no : KE00995
Synonyms
HP, BP, HAPT, HP2 ALPHA 2, HPA1S
Validation Data Gallery
Product Information
KE00995 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Haptoglobin ELISA kit is to be used to detect and quantify protein levels of endogenous human Haptoglobin. The assay recognizes human Haptoglobin. An antibody specific for human Haptoglobin has been pre-coated onto the microwells. The human Haptoglobin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Haptoglobin is added to detect the captured human Haptoglobin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Haptoglobin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Saliva |
| Assay type | Sandwich |
| Sensitivity | 0.8 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3240 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 99% | 92%-103% |
| Cell culture supernatant | 88% | 84%-90% |
| Saliva | 102% | 71%-113% |
| Urine | 102% | 77%-116% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,515.4 | 79.9 | 5.3 |
| 2 | 8 | 378.0 | 8.5 | 2.3 |
| 3 | 8 | 203.8 | 13.3 | 6.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,478.2 | 73.7 | 5.0 |
| 2 | 16 | 381.3 | 21.2 | 5.5 |
| 3 | 16 | 194.4 | 16.0 | 8.2 |
Background Information
Haptoglobin(HP), also named as zonulin, is a plasma glycoprotein and belongs to the peptidase S1 family. As a kind of acute-phase response protein, serum Hp is mainly synthesized by hepatocytes and is widely distributed in the circulation in humans. The main physiological function of serum Hp is binding free haemoglobin released from red blood cell lysis and protecting tissues and vessels from oxidative damage. The level of Hp is a useful biomarker for some diseases diagnosis, such as Hepatocellular carcinoma, Pancreatic cancer, Type 2 diabetes mellitus, Diabetes kidney disease and so on.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | HP, BP, HAPT, HP2 ALPHA 2, HPA1S |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.