Human ICAM-1 ELISA Kit
Cat no : KE00061
Synonyms
CD54, BB2, ICAM 1, ICAM1
Validation Data Gallery
Product Information
KE00061 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ICAM-1 ELISA kit is to be used to detect and quantify protein levels of endogenous ICAM-1. The assay recognizes human ICAM-1. An antibody specific for ICAM-1 has been pre-coated onto the microwells. The ICAM-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for ICAM-1 is added to detect the captured ICAM-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ICAM-1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 11.1 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3383 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 102% | 75%-126% |
| Cell culture supernatants | 94% | 86%-120% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 166.8 | 6.6 | 4.0 |
| 2 | 20 | 656.7 | 18.9 | 2.9 |
| 3 | 20 | 2,420.7 | 90.7 | 3.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 172.2 | 11.9 | 6.9 |
| 2 | 24 | 666.0 | 34.8 | 5.2 |
| 3 | 24 | 2,522.8 | 154.4 | 6.1 |
Background Information
Intercellular adhesion molecule 1 (ICAM-1, also known as CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin. It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum. Soluble form of ICAM-1 (sICAM-1) has been measured in various body fluids, with increased levels being found in serum from patients with cardiovascular disease, cancer and autoimmune diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD54, BB2, ICAM 1, ICAM1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.