Human ICAM3/CD50 ELISA Kit
Cat no : KE00633
Synonyms
CDW50, ICAM3, CD50, ICAM 3, ICAM R
Validation Data Gallery
Product Information
KE00633 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ICAM3/CD50 ELISA kit is to be used to detect and quantify protein levels of endogenous human ICAM3/CD50. The assay recognizes human ICAM3/CD50. An antibody specific for human ICAM3/CD50 has been pre-coated onto the microwells. The human ICAM3/CD50 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ICAM3/CD50 is added to detect the captured human ICAM3/CD50 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ICAM3/CD50 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3385 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 86% | 82%-90% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 9.72 | 0.18 | 1.88 |
| 2 | 8 | 2.44 | 0.03 | 1.36 |
| 3 | 8 | 1.28 | 0.04 | 2.81 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 9.42 | 0.41 | 4.40 |
| 2 | 16 | 2.32 | 0.13 | 5.80 |
| 3 | 16 | 1.26 | 0.05 | 3.89 |
Background Information
ICAM3 (Intercellular adhesion molecule 3) is also named as CD50, CDw50 and ICAM-R. ICAM3 is a member of the intercellular adhesion molecule (ICAM) family. ICAM3, being expressed constitutively on T cells and other leukocytes, is a potent signalling molecule and a major ligand in the initiation of T cell-mediated immune responses. ICAM3 is the natural ligand for both DC-SIGN and L-SIGN. ICAM3 is abundantly and constitutively expressed in all leukocytes and is probably the most important ligand for LFA-1 in initiating immune responses, and ICAM3 is a typical type I transmembrane glycoprotein. The promoter region of ICAM3 can be bound by co-transcription factors such as ETs and C/EBP, promoting macrophage maturation as well as monocyte extravasation. ICAM3 is widely expressed not only in lymphocytes such as T and B cells but also in many endothelial cells and dendritic cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CDW50, ICAM3, CD50, ICAM 3, ICAM R |