Human ICOSLG ELISA Kit
Sensitivity
2.1 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Human
Cat no : KE00326
Synonyms
B7 H2, B7 homolog 2, B7 like protein Gl50, B7 related protein 1, B7H2, B7RP 1, B7RP1, CD275, GL50, ICOS L, ICOS ligand, ICOSL, ICOSLG, KIAA0653, LICOS
Validation Data Gallery
Product Information
KE00326 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ICOSLG ELISA kit is to be used to detect and quantify protein levels of endogenous human ICOSLG. The assay recognizes human ICOSLG. An antibody specific for human ICOSLG has been pre-coated onto the microwells. The human ICOSLG protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ICOSLG is added to detect the captured human ICOSLG protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ICOSLG ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 2.1 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 23308 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 95% | 80%-109% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 503.2 | 10.7 | 2.1 |
| 2 | 20 | 117.3 | 4.2 | 3.6 |
| 3 | 20 | 27.7 | 1.8 | 6.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 495.2 | 11.2 | 2.3 |
| 2 | 24 | 122.8 | 3.5 | 2.9 |
| 3 | 24 | 33.9 | 2.7 | 7.9 |
Background Information
ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells. It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule. The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | B7 H2, B7 homolog 2, B7 like protein Gl50, B7 related protein 1, B7H2, B7RP 1, B7RP1, CD275, GL50, ICOS L, ICOS ligand, ICOSL, ICOSLG, KIAA0653, LICOS |