Human IDH1 ELISA Kit
Cat no : KE00060
Synonyms
IDCD, IDH, IDH1, IDP, IDPC, NADP(+) specific ICDH, Oxalosuccinate decarboxylase, PICD
Validation Data Gallery
Product Information
KE00060 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IDH1 ELISA kit is to be used to detect and quantify protein levels of endogenous IDH1. The assay recognizes human IDH1. An antibody specific for IDH1 has been pre-coated onto the microwells. The IDH1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IDH1 is added to detect the captured IDH1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IDH1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysates |
| Assay type | Sandwich |
| Sensitivity | 3.3 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3417 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 98% | 77%-125% |
| Cell lysates | 96% | 76%-128% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 3,400.2 | 112.2 | 3.3 |
| 2 | 20 | 726.7 | 19.2 | 2.6 |
| 3 | 20 | 176.0 | 5.4 | 3.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 3,479.4 | 96.0 | 2.8 |
| 2 | 24 | 719.6 | 20.2 | 2.8 |
| 3 | 24 | 185.8 | 5.2 | 2.8 |
Background Information
IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 may be a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway(PMID: 19359588). It is a common feature of a major subset of primary human brain cancers. IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homodimers and 50% heterodimers present in the tumor cells(PMID:21079649).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IDCD, IDH, IDH1, IDP, IDPC, NADP(+) specific ICDH, Oxalosuccinate decarboxylase, PICD |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.