Human IDS ELISA Kit
Cat no : KE00663
Synonyms
Alpha-L-iduronate sulfate sulfatase, EC:3.1.6.13, Iduronate 2-sulfatase, Iduronate 2-sulfatase 14 kDa chain, Iduronate 2-sulfatase 42 kDa chain
Validation Data Gallery
Product Information
KE00663 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human IDS ELISA kit is to be used to detect and quantify protein levels of endogenous human IDS. The assay recognizes human IDS. An antibody specific for human IDS has been pre-coated onto the microwells. The human IDS protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human IDS is added to detect the captured human IDS protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IDS ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3423 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 76% | 75%-77% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 9.05 | 0.27 | 2.98 |
| 2 | 8 | 2.51 | 0.04 | 1.79 |
| 3 | 8 | 1.34 | 0.04 | 3.29 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 8.99 | 0.46 | 5.13 |
| 2 | 16 | 2.44 | 0.11 | 4.49 |
| 3 | 16 | 1.31 | 0.06 | 4.24 |
Background Information
Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to 55 and 45 kDa mature polypeptides due to an internal proteolytic cleavage.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Alpha-L-iduronate sulfate sulfatase, EC:3.1.6.13, Iduronate 2-sulfatase, Iduronate 2-sulfatase 14 kDa chain, Iduronate 2-sulfatase 42 kDa chain |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.