Human IFN-gamma ELISA Kit

Sensitivity

1.0 pg/mL

Range

15.6-1000 pg/mL

Reacted Species

Human

Cat no : KE00063

Synonyms

IFNG



Product Information

KE00063 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IFN-gamma ELISA kit is to be used to detect and quantify protein levels of endogenous IFN-gamma. The assay recognizes human IFN-gamma. An antibody specific for IFN-gamma has been pre-coated onto the microwells. The IFN-gamma protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IFN-gamma is added to detect the captured IFN-gamma protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Human IFN-gamma ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 1.0 pg/mL
Range 15.6-1000 pg/mL
Reacted Species Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human plasma95%86%-102%
Cell culture supernatants111%84%-121%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120688.038.05.5
220202.414.87.3
32051.03.56.9

InterAssay

Samplenmean ( pg/mL)SDCV%
124927.079.08.5
224259.720.78.0
32466.04.26.4

Background Information

Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms IFNG

Publications

SpeciesTitle
human

Commun Biol

Arginine GlcNAcylation of Rab small GTPases by the pathogen Salmonella Typhimurium.

Authors - Kun Meng

Oncol Lett

Effect of combined propofol-sevoflurane anesthesia on immune function in pediatric patients with acute lymphoblastic leukemia.

Authors - Niu Di
human

Climacteric

Evaluation of CD4+CD25+FOXP3+ regulatory T cells and FOXP3 mRNA in premature ovarian insufficiency.

Authors - J Xiong