Human IGF1R ELISA Kit
Cat no : KE00798
Synonyms
IGF 1 receptor, IGF-I receptor, IGFIR, IGFR, CD221
Validation Data Gallery
Product Information
KE00798 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human IGF1R ELISA kit is to be used to detect and quantify protein levels of endogenous human IGF1R. The assay recognizes human IGF1R. An antibody specific for human IGF1R has been pre-coated onto the microwells. The human IGF1R protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human IGF1R is added to detect the captured human IGF1R protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IGF1R ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.07 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3480 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 96% | 77%-109% |
| Cell lysate | 91% | 81%-98% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 9.94 | 0.35 | 3.53 |
| 2 | 8 | 2.51 | 0.12 | 4.75 |
| 3 | 8 | 1.25 | 0.09 | 7.55 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 9.34 | 0.67 | 7.19 |
| 2 | 16 | 2.33 | 0.22 | 9.46 |
| 3 | 16 | 1.19 | 0.09 | 7.39 |
Background Information
IGF1R (Insulin-like Growth Factor 1 Receptor) is a transmembrane receptor tyrosine kinase. It plays a critical role in growth, development, metabolism, and disease pathogenesis. IGF1R is a heterotetramer composed of two extracellular α-subunits (ligand-binding) and two transmembrane β-subunits (tyrosine kinase domain). IGF1R regulates adipocyte development and lipid storage. Adipocyte-specific knockout increases circulating IGF-1 levels.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IGF 1 receptor, IGF-I receptor, IGFIR, IGFR, CD221 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.