Human IGFBP2 ELISA Kit
Cat no : KE00835
Synonyms
BP 2, BP2, IBP 2, IBP2, IBP-2
Validation Data Gallery
Product Information
KE00835 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human IGFBP2 ELISA kit is to be used to detect and quantify protein levels of endogenous human IGFBP2. The assay recognizes human IGFBP2. An antibody specific for human IGFBP2 has been pre-coated onto the microwells. The human IGFBP2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human IGFBP2 is added to detect the captured human IGFBP2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IGFBP2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine |
| Assay type | Sandwich |
| Sensitivity | 6.2 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3485 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 88% | 71%-109% |
| Cell culture supernatant | 86% | 75%-96% |
| Urine | 83% | 73%-97% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,338.3 | 64.7 | 4.8 |
| 2 | 8 | 286.1 | 3.7 | 1.3 |
| 3 | 8 | 154.3 | 3.4 | 2.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,287.1 | 81.9 | 6.4 |
| 2 | 16 | 295.0 | 10.8 | 3.7 |
| 3 | 16 | 156.3 | 3.6 | 2.3 |
Background Information
Insulin-like growth factor (IGF) binding protein 2 (IGFBP2) was discovered and identified as an IGF system regulator, controlling the distribution, function, and activity of IGFs in the pericellular space. IGFBP2 is a developmentally regulated gene that is highly expressed in embryonic and fetal tissues and markedly decreases after birth. IGFBP2 is a secreted protein which prevents IGF-I/-II binding to their receptors, and it is also implicated in the regulation of tumor microenvironment (TME) in a macrophage-dependent manner. Serum IGFBP2 level is recognized as a diagnostic biomarker of severe malnutrition in patients with advanced lung cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BP 2, BP2, IBP 2, IBP2, IBP-2 |