Speedy™ Human IGFBP2 One-Step ELISA Kit
Cat no : SE50080
Synonyms
BP 2, BP2, IBP 2, IBP2, IBP-2
Validation Data Gallery
Product Information
| Product name | Speedy™ Human IGFBP2 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine |
| Assay type | Sandwich |
| Sensitivity | 15.4 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3485 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 87% | 83%-93% |
| Cell culture supernatant | 93% | 85%-102% |
| Urine | 93% | 81%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,254.9 | 45.8 | 3.6 |
| 2 | 8 | 341.4 | 10.0 | 2.9 |
| 3 | 8 | 182.0 | 6.7 | 3.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,272.1 | 50.2 | 3.9 |
| 2 | 16 | 344.9 | 10.0 | 2.9 |
| 3 | 16 | 183.1 | 7.2 | 3.9 |
Background Information
Insulin-like growth factor (IGF) binding protein 2 (IGFBP2) was discovered and identified as an IGF system regulator, controlling the distribution, function, and activity of IGFs in the pericellular space. IGFBP2 is a developmentally regulated gene that is highly expressed in embryonic and fetal tissues and markedly decreases after birth. IGFBP2 is a secreted protein which prevents IGF-I/-II binding to their receptors, and it is also implicated in the regulation of tumor microenvironment (TME) in a macrophage-dependent manner. Serum IGFBP2 level is recognized as a diagnostic biomarker of severe malnutrition in patients with advanced lung cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BP 2, BP2, IBP 2, IBP2, IBP-2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.