Human IGFBP7 ELISA Kit
Cat no : KE00373
Synonyms
IGFBP7,IBP7,IBP-7,IGF-binding protein 7,IGFBP-7
Validation Data Gallery
Product Information
KE00373 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human IGFBP7 ELISA kit is to be used to detect and quantify protein levels of endogenous human IGFBP7. The assay recognizes human IGFBP7. An antibody specific for human IGFBP7 has been pre-coated onto the microwells. The human IGFBP7 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human IGFBP7 is added to detect the captured human IGFBP7 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IGFBP7 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3490 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Urine | 87% | 71%-100% |
| Cell culture supernatant | 87% | 81%-102% |
| Human serum | 111% | 88%-128% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 12.53 | 0.88 | 7.04 |
| 2 | 20 | 2.40 | 0.14 | 5.69 |
| 3 | 20 | 0.57 | 0.05 | 8.09 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 10.06 | 0.94 | 9.34 |
| 2 | 24 | 2.35 | 0.10 | 4.42 |
| 3 | 24 | 0.66 | 0.05 | 7.28 |
Background Information
IGFBP7, also named as MAC25, PSF, IBP-7 and TAF,is a member of the insulin-like growth factor (IGF)-binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. It also stimulates prostacyclin production and cell adhesion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and one variant has been associated with retinal arterial macroaneurysm.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IGFBP7,IBP7,IBP-7,IGF-binding protein 7,IGFBP-7 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.