Human/Cynomolgus Monkey IL-1 beta ELISA Kit
Cat no : KE00592
Synonyms
Catabolin, IL 1, IL 1 beta, IL1 beta, IL1- beta
Validation Data Gallery
Product Information
KE00592 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/cynomolgus monkey IL-1 beta ELISA kit is to be used to detect and quantify protein levels of endogenous human/cynomolgus monkey IL-1 beta. The assay recognizes human/cynomolgus monkey IL-1 beta. An antibody specific for human/cynomolgus monkey IL-1 beta has been pre-coated onto the microwells. The human/cynomolgus monkey IL-1 beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/cynomolgus monkey IL-1 beta is added to detect the captured human/cynomolgus monkey IL-1 beta protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Cynomolgus Monkey IL-1 beta ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma,Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 4.7-300 pg/mL |
| Reactivity | Human/Cynomolgus Monkey |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3553 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 82% | 71%-94% |
| Cell culture supernatants | 91% | 88%-95% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 86.9 | 1.9 | 2.2 |
| 2 | 8 | 22.1 | 0.8 | 3.6 |
| 3 | 8 | 11.8 | 0.8 | 6.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 142.3 | 3.4 | 2.4 |
| 2 | 16 | 34.7 | 1.5 | 4.4 |
| 3 | 16 | 17.3 | 0.7 | 3.8 |
Background Information
Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1 beta and their naturally occurring inhibitor IL-1RN. Interleukin 1 beta (IL-1 beta), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1 beta is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1 beta is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Catabolin, IL 1, IL 1 beta, IL1 beta, IL1- beta |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.