Speedy™ Human IL-1R2 One-Step ELISA Kit
Cat no : SE50057
Synonyms
IL1R2, CD121 antigen-like family member B, CD121b, IL 1R 2, IL 1R beta
Validation Data Gallery
Product Information
| Product name | Speedy™ Human IL-1R2 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 7.0 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7850 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatant | 107% | 101%-112% |
| Human plasma | 108% | 103%-126% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,016.6 | 18.3 | 1.8 |
| 2 | 8 | 269.4 | 6.3 | 2.3 |
| 3 | 8 | 143.8 | 3.3 | 2.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 999.3 | 34.8 | 3.5 |
| 2 | 16 | 262.6 | 10.4 | 4.0 |
| 3 | 16 | 138.0 | 6.7 | 4.9 |
Background Information
Interleukin-1 receptor type 2 (IL-1R2, also known as CD121b) is a receptor for the pro-inflammatory cytokines IL-1α and IL-1β. It plays a crucial role in the negative regulation of the IL-1 signaling pathway, which is involved in inflammation and immune responses. IL-1R2 also regulates IL-1 maturation and cell activation, promotes cell survival, and participates in IL-1-dependent internalization. Soluble IL-1R2 (sIL-1R2) acts as a decoy receptor by binding to IL-1α and IL-1β, preventing their interaction with IL-1R1 and IL-1RAP. This inhibits the activation of the IL-1 signaling pathway, thereby reducing inflammation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL1R2, CD121 antigen-like family member B, CD121b, IL 1R 2, IL 1R beta |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.