Speedy™ Human IL-3 One-Step ELISA Kit
Cat no : SE50042
Synonyms
IL3, IL 3, Interleukin 3, Interleukin-3, Multipotential colony-stimulating factor
Validation Data Gallery
Product Information
| Product name | Speedy™ Human IL-3 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 7.3 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3562 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 89%-109% |
| Cell culture supernatant | 108% | 98%-122% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,049.1 | 16.3 | 1.6 |
| 2 | 8 | 259.4 | 2.8 | 1.1 |
| 3 | 8 | 73.8 | 1.6 | 2.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,051.2 | 13.2 | 1.3 |
| 2 | 16 | 261.0 | 4.3 | 1.6 |
| 3 | 16 | 76.9 | 3.4 | 4.4 |
Background Information
Interleukin-3 (IL-3) is a multilineage hematopoietic growth factor that promotes the proliferation, differentiation and survival of early multilineage hematopoietic progenitors. In particular, this cytokine plays a key role in stimulating the proliferation and survival of myeloid precursors. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL3, IL 3, Interleukin 3, Interleukin-3, Multipotential colony-stimulating factor |
FAQ
1、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.