Speedy™ Human/Cynomolgus Monkey IL-4 One-Step ELISA Kit
Cat no : SE50018
Synonyms
IL4, B-cell stimulatory factor 1, BSF-1, IFN-beta-2, IL 4
Validation Data Gallery
Product Information
| Product name | Speedy™ Human/Cynomolgus Monkey IL-4 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum, Human plasma, Cynomolgus monkey serum, Cynomolgus monkey plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.9 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human/Cynomolgus Monkey |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3565 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 86% | 80%-93% |
| Cell culture supernatant | 107% | 90%-116% |
| Cynomolgus monkey serum | 98% | 89%-106% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,092.3 | 18.7 | 1.7 |
| 2 | 8 | 253.7 | 7.8 | 3.1 |
| 3 | 8 | 120.6 | 2.1 | 1.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,069.6 | 35.1 | 3.3 |
| 2 | 16 | 249.7 | 8.1 | 3.2 |
| 3 | 16 | 118.1 | 3.5 | 3.0 |
Background Information
Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4 + T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL4, B-cell stimulatory factor 1, BSF-1, IFN-beta-2, IL 4 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.