Human IL-10 ELISA Kit

Sensitivity

0.5 pg/mL

Range

7.8-500 pg/mL

Reacted Species

Human

Cat no : KE00012

Synonyms

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF



Product Information

KE00012 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL10 ELISA kit is to be used to detect and quantify protein levels of endogenous IL10. The assay recognizes human IL10. An antibody specific for IL10 has been pre-coated onto the microwells. The IL10 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IL10 is added to detect the captured IL10 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Human IL-10 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 0.5 pg/mL
Range 7.8-500 pg/mL
Reacted Species Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human plamsa100%80%-127%
Cell culture supernatants101%87%-114%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12038.91.74.3
220133.47.05.2
320484.917.13.5

InterAssay

Samplenmean ( pg/mL)SDCV%
12439.92.46.1
224137.57.85.7
32452739.77.5

Background Information

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF

Publications

SpeciesSample TypeTitle
humanserum

Exp Ther Med

Changes in expression of p53 and inflammatory factors in patients with ulcerative colitis.

Authors - Hui Su
humanserum

Antibiotics (Basel)

Rifaximin Alleviates Endotoxemia with Decreased Serum Levels of Soluble CD163 and Mannose Receptor and Partial Modification of Gut Microbiota in Cirrhotic Patients.

Authors - Kosuke Kaji
plasma

J Nutr Biochem

Can the dietary fat type facilitate memory impairments in adulthood? A comparative study between Mediterranean and Western-based diet in rats.

Authors - Priscila Haygert
humancell culture supernatants

Biomed Pharmacother

Total glucosides of paeony improves the immunomodulatory capacity of MSCs partially via the miR-124/STAT3 pathway in oral lichen planus.

Authors - Zhongfang Zhao
humancell culture supernatants

Int J Mol Med

Hypoxic mast cells accelerate the proliferation, collagen accumulation and phenotypic alteration of human lung fibroblasts.

Authors - Xiang Wang
humanserum

J Periodontol

Periodontitis is associated with systemic inflammation and vascular endothelial dysfunction in lacunar infarct patients.

Authors - Yago Leira