Human IL-15 ELISA Kit
Cat no : KE00102
Synonyms
IL 15, IL15, IL-15, interleukin 15, interleukin-15
Validation Data Gallery
Product Information
KE00102 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL15 ELISA kit is to be used to detect and quantify protein levels of endogenous IL15. The assay recognizes human IL15. An antibody specific for IL15 has been pre-coated onto the microwells. The IL15 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL15 is added to detect the captured IL15 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-15 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.9 pg/mL |
| Range | 3.9 - 250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3600 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 93% | 72%-112% |
| Human plasma | 104% | 92%-116% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 20.9 | 0.9 | 4.1 |
| 2 | 20 | 81.8 | 4.2 | 5.1 |
| 3 | 20 | 309.1 | 15.5 | 5.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 21.4 | 1.5 | 7.2 |
| 2 | 24 | 77.4 | 5.2 | 6.8 |
| 3 | 24 | 274.4 | 23.9 | 8.7 |
Background Information
Interleukin 15 (IL15) is a cytokine important for the development, maturation, and function of many cells of the immune system including NK, NKT, gammadeltaT, and CD8(+) T cells. Its expression affects homeostasis and function of B cells through NK cell-derived IFNG. IL-15 binds a trimeric IL-15 receptor (IL-15R) complex formed by a specific receptor chain (IL-15Rα) combined with theβandγchains of the IL-2R, acting as signal transducing components. High levels of IL15 expression have been associated with the pathogenesis of autoimmune and inflammatory diseases like Crohn's disease, psoriasis, leukemias, rheumatoid arthritis (RA) and graft rejection, resulting in the IL15 system being a target of interest for the treatment of these diseases
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL 15, IL15, IL-15, interleukin 15, interleukin-15 |
Publications
| Species | Sample Type | Title |
|---|---|---|
J Med Virol Combination of novel oncolytic herpesvirus with paclitaxel as an efficient strategy for breast cancer therapy |