Human IL17RA ELISA Kit
Cat no : KE00788
Synonyms
CD217, CANDF5, CDw217, hIL 17R, IL 17 receptor A
Validation Data Gallery
Product Information
KE00788 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human IL17RA ELISA kit is to be used to detect and quantify protein levels of endogenous human IL17RA. The assay recognizes human IL17RA. An antibody specific for human IL17RA has been pre-coated onto the microwells. The human IL17RA protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human IL17RA is added to detect the captured human IL17RA protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL17RA ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 23765 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 112% | 107%-119% |
| Human serum | 93% | 90%-97% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,207.8 | 53.7 | 4.4 |
| 2 | 8 | 317.9 | 18.0 | 5.7 |
| 3 | 8 | 149.4 | 5.9 | 4.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,208.0 | 48.4 | 4.0 |
| 2 | 16 | 318.0 | 19.6 | 6.2 |
| 3 | 16 | 147.7 | 6.3 | 4.2 |
Background Information
IL17 is a proinflammatory cytokine. IL17 can signal as homodimers or heterodimers through a heterodimeric receptor formed by IL17 receptor A (IL17RA) and IL17 receptor C (IL17RC) subunits. IL17RA is ubiquitously expressed in both the epithelial and hematopoietic cell compartments. IL17/IL17RA signaling plays an important physiological role in regulating the mucosal host defense against invading microorganisms, but it can also play pathogenic roles in several inflammatory or autoimmune conditions; furthermore, it has been described as pro-tumorigenic in several cancer types.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD217, CANDF5, CDw217, hIL 17R, IL 17 receptor A |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Front Immunol Small extracellular vesicles proteome reveals persistent inflammatory and coagulopathic dysregulation in long-COVID. |