Human IL18BP ELISA Kit
Cat no : KE00454
Synonyms
IL 18BP, IL-18BP, IL18BPa, interleukin 18 binding protein, Interleukin-18-binding protein
Validation Data Gallery
Product Information
KE00454 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human IL18BP ELISA kit is to be used to detect and quantify protein levels of endogenous human IL18BP. The assay recognizes human IL18BP. An antibody specific for human IL18BP has been pre-coated onto the microwells. The human IL18BP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human IL18BP is added to detect the captured human IL18BP protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL18BP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine |
| Assay type | Sandwich |
| Sensitivity | 0.08 ng/mL |
| Range | 0.234-15 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10068 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 94%-102% |
| Urine | 99% | 91%-109% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 7.14 | 0.22 | 3.15 |
| 2 | 8 | 2.06 | 0.04 | 1.89 |
| 3 | 8 | 1.21 | 0.04 | 3.71 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 7.08 | 0.19 | 2.68 |
| 2 | 16 | 2.05 | 0.04 | 2.12 |
| 3 | 16 | 1.22 | 0.04 | 3.27 |
Background Information
IL-18 Binding Protein (IL18BP) is a soluble protein that acts as a natural antagonist of interleukin-18 (IL-18), a pro-inflammatory cytokine involved in immune responses. It binds to IL-18 with high affinity, preventing IL-18 from interacting with its receptors (IL-18Rα and IL-18Rβ) and thus inhibiting its biological activity. This regulation is crucial for maintaining immune homeostasis and preventing excessive inflammation. IL18BP is a biomarker for disease activity and severity, as well as a therapeutic target in cancer immunotherapy.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL 18BP, IL-18BP, IL18BPa, interleukin 18 binding protein, Interleukin-18-binding protein |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.