Human IL-1R1 ELISA Kit
Cat no : KE00116
Synonyms
IL 1R 1, IL1R, IL1R1, IL-1R1, IL1RT1, interleukin 1 receptor, type I, P80
Validation Data Gallery
Product Information
KE00116 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL1R1 ELISA kit is to be used to detect and quantify protein levels of endogenous IL1R1. The assay recognizes human IL1R1. An antibody specific for IL1R1 has been pre-coated onto the microwells. The IL1R1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL1R1 is added to detect the captured IL1R1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-1R1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 5.0 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3554 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 98% | 94%-105% |
| Cell culture supernatants | 87% | 81%-92% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 541.8 | 21.2 | 3.9 |
| 2 | 20 | 1,260.9 | 75.0 | 5.9 |
| 3 | 20 | 2,346.7 | 117.9 | 5.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 558.4 | 22.8 | 4.1 |
| 2 | 24 | 1,147.1 | 56.9 | 5.0 |
| 3 | 24 | 2,271.2 | 59.9 | 2.6 |
Background Information
Interlukin-1 polypeptides are pleiotropic cytokines exerting diverse functions in different tissues. IL-1R1 (also known as CD121a) is a receptor for interleukin-1 alpha (IL-1α), interleukin-1 beta (IL-1β), and interleukin-1 receptor antagonist (IL-1Ra). Human IL-1R1 was first cloned from human T cells. This receptor contains a large cytoplasmic region and an extracellular, IL-1 binding portion composed of three immunoglobulin-like domains. IL-1R1 expression has been found in many cell types including T cells, macrophages, neutrophils, eosinophils, basophils, mast cells, fibroblasts, and endothelial cells. IL-1R1 can be shed from the cell membrane as a soluble form (sIL-1R1) which can function as a soluble decoy receptor and prevent the binding of IL-1α, IL-1β, and IL-1Ra to membrane IL-1R1.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL 1R 1, IL1R, IL1R1, IL-1R1, IL1RT1, interleukin 1 receptor, type I, P80 |