Human IL-2 ELISA Kit

Sensitivity

3.6 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Human

Cat no : KE00017

Synonyms

Aldesleukin, IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF



Product Information

KE00017 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-2 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-2. The assay recognizes human IL-2. An antibody specific for IL-2 has been pre-coated onto the microwells. The IL-2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-2 is added to detect the captured IL-2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human IL-2 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 3.6 pg/mL
Range 15.6-1000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Cell culture supernatants113%103%-121%
Human plasma92%81%-107%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1201109.735.83.2
220339.315.74.6
32070.82.02.9

InterAssay

Samplenmean ( pg/mL)SDCV%
1241370.3126.49.2
224420.632.57.7
32479.96.58.1

Background Information

Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of theIL-2Rβand IL-2Rγreceptor subunits.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Aldesleukin, IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF

Publications

SpeciesSample TypeTitle
humancell culture supernatants

ACS Appl Bio Mater

Isolation of DNA Aptamer Targeting PD-1 with an Antitumor Immunotherapy Effect.

Authors - Tian Gao
humancell culture supernatants

Biomed Mater

Aptamer-functionalized targeted siRNA delivery system for tumor immunotherapy.

Authors - Haiyin Lv
humancell culture supernatants

Biomed Mater

Aptamer-functionalized targeted siRNA delivery system for tumor immunotherapy.

Authors - Haiyin Lv
humancell culture supernatants

Front Immunol

Co-Expression of miR155 or LSD1 shRNA Increases the Anti-Tumor Functions of CD19 CAR-T Cells.

Authors - Jing Zhang