Human IL-2 ELISA Kit
Sensitivity
3.6 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Human
Cat no : KE00017
Synonyms
Aldesleukin, IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF
Validation Data Gallery
Product Information
KE00017 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-2 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-2. The assay recognizes human IL-2. An antibody specific for IL-2 has been pre-coated onto the microwells. The IL-2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-2 is added to detect the captured IL-2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 3.6 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 113% | 103%-121% |
| Human plasma | 92% | 81%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1109.7 | 35.8 | 3.2 |
| 2 | 20 | 339.3 | 15.7 | 4.6 |
| 3 | 20 | 70.8 | 2.0 | 2.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1370.3 | 126.4 | 9.2 |
| 2 | 24 | 420.6 | 32.5 | 7.7 |
| 3 | 24 | 79.9 | 6.5 | 8.1 |
Background Information
Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of theIL-2Rβand IL-2Rγreceptor subunits.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Aldesleukin, IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF |