Product name:

Human IL2 ELISA Kit

Tests:

1 X 96 well plate

Sample type:

Serum, Plasma, Cell culture supernatant

Assay type:

Sandwich

Sensitivity:

3.6 pg/mL

Range:

15.6-1000 pg/mL

Reacted Species:

Human

Tested applications:

Sandwich ELISA

Recovery:

Sample Type	Average	Range
Plasma	92%	81%-107%
Cell culture supernatant	112%	103%-121%

Product overview:

KE00017 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL2 ELISA kit is to be used to detect and quantify protein levels of endogenous IL2. The assay recognizes human IL2. A monoclonal antibody specific for IL2 has been pre-coated onto the microwells. The IL2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for IL2 is added to detect the captured IL2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm and 630 nm.

Storage Instructions:

Store standard and Plate at -20° the rests store at 4°.Refer to the protocol for further storage instructions

Synonyms:

Aldesleukin, IL 2, interleukin 2, lymphokine, T cell growth factor, TCGF