Human IL-2RA ELISA Kit
Cat no : KE00140
Synonyms
CD25, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA
Validation Data Gallery
Product Information
KE00140 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL2RA ELISA kit is to be used to detect and quantify protein levels of endogenous IL2RA. The assay recognizes human IL2RA. An antibody specific for IL2RA has been pre-coated onto the microwells. The IL2RA protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human IL2RA is added to detect the captured human IL2RA protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-2RA ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 5.8 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3559 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 92% | 70%-125% |
| Cell culture supernatants | 101% | 86%-113% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,100.3 | 208.2 | 9.9 |
| 2 | 20 | 553.1 | 48.6 | 8.8 |
| 3 | 20 | 129.1 | 11.1 | 8.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,486.2 | 237.1 | 9.5 |
| 2 | 24 | 616.5 | 48.4 | 7.9 |
| 3 | 24 | 136.2 | 12.4 | 9.1 |
Background Information
Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. Soluble IL-2R alpha has been isolated and determined to result from extracellular proteolyisis. Soluble IL-2R alpha has been found in the plasma of patients with certain hematologic malignancies. Elevated plasma or serum concentration of soluble IL-2R alpha has also been reported as a biomarker of immune activation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD25, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.