Human IL-3 ELISA Kit
Cat no : KE00185
Synonyms
Hematopoietic growth factor, IL 3, IL3, IL-3, Interleukin 3, Mast cell growth factor, MCGF, MULTI CSF, P cell stimulating factor
Validation Data Gallery
Product Information
KE00185 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-3 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-3. The assay recognizes human IL-3. An antibody specific for IL-3 has been pre-coated onto the microwells. The IL-3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL-3 is added to detect the captured IL-3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.18 pg/mL |
| Range | 7.8 - 500 pg/mL, 15.6 - 1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3562 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 82% | 72%-97% |
| Cell culture supernatants | 84% | 73%-95% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 256.0 | 4.4 | 1.7 |
| 2 | 20 | 62.8 | 0.8 | 1.3 |
| 3 | 20 | 14.4 | 0.5 | 3.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 270.1 | 6.6 | 2.4 |
| 2 | 24 | 63.8 | 1.9 | 3.0 |
| 3 | 24 | 15.1 | 0.8 | 5.0 |
Background Information
Interleukin-3 (IL-3) is a multilineage hematopoietic growth factor that promotes the proliferation, differentiation and survival of early multilineage hematopoietic progenitors. In particular, this cytokine plays a key role in stimulating the proliferation and survival of myeloid precursors. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Hematopoietic growth factor, IL 3, IL3, IL-3, Interleukin 3, Mast cell growth factor, MCGF, MULTI CSF, P cell stimulating factor |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.