Human IL-31 ELISA Kit
Cat no : KE00086
Synonyms
IL 31, IL31, IL-31, interleukin 31
Validation Data Gallery
Product Information
KE00086 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Human IL31 ELISA kit is to be used to detect and quantify protein levels of endogenous human IL31. The assay recognizes human IL31. An antibody specific for human IL31 has been pre-coated onto the microwells. The human IL31 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human IL31 is added to detect the captured human IL31 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-31 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 7.4 pg/mL |
| Range | 31.25 - 2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 386653 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 91% | 74%-117% |
| Cell culture supernatants | 91% | 83%-113% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 41.0 | 1.7 | 4.2 |
| 2 | 20 | 431.6 | 10.7 | 2.5 |
| 3 | 20 | 1,731.5 | 52.7 | 3.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 37.4 | 3.3 | 8.9 |
| 2 | 24 | 415.9 | 15.1 | 3.6 |
| 3 | 24 | 1,726.2 | 74.8 | 4.3 |
Background Information
Interleukin-31 (IL-31) is a newly discovered four-helix bundle cytokine and expressed by several types of cells under both normal and disease state, such as activated CD4+T cells, mast cells, monocytes, macrophages, immature and mature monocyte-derived dendritic cells and so on. IL-31 signals through a heterodimeric receptor complex consisting of the IL31 receptor alpha (IL31Rα) and the oncostatin M receptor (OSMR). IL-31 plays a predominant function in the mediation of inflammatory and lymphoma-associated itch. Increased IL-31 expression has been detected in inflamed tissues from patients with atopic dermatitis, bowel diseases, allergic asthma and rhinitis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL 31, IL31, IL-31, interleukin 31 |