Human IMPDH1 ELISA Kit
Cat no : KE00935
Synonyms
EC:1.1.1.205, IMP dehydrogenase 1, IMPD 1, IMPD1, IMPDH 1
Validation Data Gallery
Product Information
KE00935 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human IMPDH1 ELISA kit is to be used to detect and quantify protein levels of endogenous human IMPDH1. The assay recognizes human IMPDH1. An antibody specific for human IMPDH1 has been pre-coated onto the microwells. The human IMPDH1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human IMPDH1 is added to detect the captured human IMPDH1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IMPDH1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.20 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3614 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 86% | 80%-90% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 21.52 | 0.92 | 4.27 |
| 2 | 8 | 5.21 | 0.09 | 1.74 |
| 3 | 8 | 2.68 | 0.08 | 3.07 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 20.77 | 1.30 | 6.24 |
| 2 | 16 | 5.21 | 0.09 | 1.68 |
| 3 | 16 | 2.64 | 0.09 | 3.58 |
Background Information
IMPDH1 (inosine monophosphate dehydrogenase 1) is a key enzyme involved in the de novo synthesis pathway of purine nucleotides. It catalyzes the conversion of inosine monophosphate (IMP) to xanthosine monophosphate (XMP), which is a rate-limiting step in the synthesis of guanosine monophosphate (GMP). IMPDH1 exists as a tetramer within cells and is crucial for maintaining the balance of the intracellular guanine nucleotide pool. This balance affects processes related to cell proliferation, including DNA and RNA synthesis, signal transduction, and energy transfer. Moreover, abnormal expression of IMPDH1 is associated with various diseases, including cancer and autoimmune diseases. In the retina, mutations in IMPDH1 are linked to autosomal dominant retinitis pigmentosa (adRP) type RP10. These mutations primarily affect the allosteric regulation of the enzyme rather than its catalytic activity, leading to disruptions in guanine nucleotide metabolism in the retina.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:1.1.1.205, IMP dehydrogenase 1, IMPD 1, IMPD1, IMPDH 1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.