Human INSR ELISA Kit
Cat no : KE00630
Synonyms
CD220, EC:2.7.10.1, HHF5, insulin receptor, IR
Validation Data Gallery
Product Information
KE00630 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human INSR ELISA kit is to be used to detect and quantify protein levels of endogenous human INSR. The assay recognizes human INSR. An antibody specific for human INSR has been pre-coated onto the microwells. The human INSR protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human INSR is added to detect the captured human INSR protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human INSR ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3643 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 95% | 86%-102% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 26.83 | 1.23 | 4.58 |
| 2 | 8 | 5.71 | 0.29 | 5.13 |
| 3 | 8 | 2.89 | 0.14 | 4.98 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 27.51 | 1.73 | 6.30 |
| 2 | 16 | 5.79 | 0.31 | 5.29 |
| 3 | 16 | 2.92 | 0.16 | 5.46 |
Background Information
INSR (Insulin Receptor) is a transmembrane receptor tyrosine kinase, composed of two α subunits and two β subunits linked by disulfide bonds. The α subunits are located extracellularly and contain the insulin-binding sites, while the β subunits span the cell membrane and contain the intracellular tyrosine kinase domains. INSR is a critical protein involved in regulating glucose homeostasis and metabolic processes. The binding of INS to the receptor's extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response. In addition to the widely active Sp1 factor, tissue-specific transcription factors have also been implicated in the regulation of the INSR promoter.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD220, EC:2.7.10.1, HHF5, insulin receptor, IR |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.