Human INTS6 ELISA Kit
Cat no : KE00468
Synonyms
INTS6,DBI 1,DBI1,DDX26,DDX26A
Validation Data Gallery
Product Information
KE00468 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human INTS6 ELISA kit is to be used to detect and quantify protein levels of endogenous human INTS6. The assay recognizes human INTS6. An antibody specific for human INTS6 has been pre-coated onto the microwells. The human INTS6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human INTS6 is added to detect the captured human INTS6 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human INTS6 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 26512 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 92% | 85%-101% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2.50 | 0.07 | 2.91 |
| 2 | 8 | 0.97 | 0.04 | 4.01 |
| 3 | 8 | 0.52 | 0.02 | 3.75 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2.53 | 0.07 | 2.57 |
| 2 | 16 | 1.00 | 0.05 | 4.54 |
| 3 | 16 | 0.53 | 0.02 | 3.31 |
Background Information
INTS6 is a component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. INTS6 is widely expressed in various tissues, including the testis and thyroid, and has important biological functions in transcriptional regulation, phosphatase activity regulation, and disease occurrence.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | INTS6,DBI 1,DBI1,DDX26,DDX26A |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.