Human IRF9 ELISA Kit
Cat no : KE00898
Synonyms
IFN-alpha-responsive transcription factor subunit, Interferon-stimulated gene factor 3 gamma, IRF 9, IRF-9, ISGF-3 gamma
Validation Data Gallery
Product Information
KE00898 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human IRF9 ELISA kit is to be used to detect and quantify protein levels of endogenous human IRF9. The assay recognizes human IRF9. An antibody specific for human IRF9 has been pre-coated onto the microwells. The human IRF9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human IRF9 is added to detect the captured human IRF9 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IRF9 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.17 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10379 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 87% | 79%-92% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 26.93 | 1.57 | 5.82 |
| 2 | 8 | 6.47 | 0.39 | 5.97 |
| 3 | 8 | 3.34 | 0.20 | 5.89 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 26.57 | 1.72 | 6.49 |
| 2 | 16 | 6.21 | 0.47 | 7.49 |
| 3 | 16 | 3.11 | 0.35 | 11.10 |
Background Information
IRF9, also known as Interferon Regulatory Factor 9, is a key transcriptional regulator that mediates interferon (IFN) signaling as part of the ISGF3 (IFN-stimulated gene factor 3) complex (along with STAT1 and STAT2). It binds to interferon-stimulated response elements (ISREs) in DNA, activating the expression of IFN-stimulated genes (ISGs) critical for antiviral defense, immune regulation, and tumor suppression. IRF9 plays a central role in the JAK-STAT signaling pathway, particularly in response to type I and type III IFNs. Dysregulation of IRF9 has been linked to autoimmune diseases, viral infections, and cancer, highlighting its importance in immune and inflammatory responses.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IFN-alpha-responsive transcription factor subunit, Interferon-stimulated gene factor 3 gamma, IRF 9, IRF-9, ISGF-3 gamma |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.