Human IVNS1ABP ELISA Kit
Cat no : KE00926
Synonyms
ARA3, Aryl hydrocarbon receptor-associated protein 3, FLARA3, HSPC068, Influenza virus NS1A-binding protein
Validation Data Gallery
Product Information
KE00926 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human IVNS1ABP ELISA kit is to be used to detect and quantify protein levels of endogenous human IVNS1ABP. The assay recognizes human IVNS1ABP. An antibody specific for human IVNS1ABP has been pre-coated onto the microwells. The human IVNS1ABP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human IVNS1ABP is added to detect the captured human IVNS1ABP protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IVNS1ABP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.07 ng/mL |
| Range | 0.39-25 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10625 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 87% | 84%-90% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 57.16 | 3.55 | 6.21 |
| 2 | 8 | 13.72 | 1.11 | 8.08 |
| 3 | 8 | 6.77 | 0.25 | 3.68 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 56.53 | 4.28 | 7.57 |
| 2 | 16 | 13.30 | 0.96 | 7.20 |
| 3 | 16 | 6.74 | 0.36 | 5.39 |
Background Information
IVNS1ABP can stabilize F-actin, regulate cytoskeleton dynamics, and participate in cell division, cell motility, and phagocytosis. In addition, IVNS1ABP is also involved in pre-mRNA splicing, the aryl hydrocarbon receptor (AHR) pathway, protein ubiquitination, and viral gene expression regulation, such as affecting viral replication by inhibiting host pre-mRNA splicing and promoting influenza virus M2 protein production.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ARA3, Aryl hydrocarbon receptor-associated protein 3, FLARA3, HSPC068, Influenza virus NS1A-binding protein |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.