Speedy™ Human IgA One-Step ELISA Kit
Cat no : SE50339
Synonyms
Human IgA, Human IgA Heavy Chain
Validation Data Gallery
Product Information
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 106% | 101%-111% |
| Urine | 95% | 89%-98% |
| Saliva | 97% | 88%-117% |
| Human milk | 97% | 92%-101% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 9.39 | 0.48 | 5.11 |
| 2 | 8 | 2.58 | 0.19 | 7.36 |
| 3 | 8 | 1.36 | 0.02 | 1.47 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 9.39 | 0.40 | 4.26 |
| 2 | 16 | 2.58 | 0.14 | 5.43 |
| 3 | 16 | 1.39 | 0.04 | 2.88 |
Background Information
Immunoglobulin A (IgA) is a crucial antibody that plays a pivotal role in the immune system's first line of defense, primarily safeguarding the body's mucosal surfaces-such as the respiratory, gastrointestinal, and urogenital tracts. As the second most abundant isotype in human serum and the dominant antibody found in external secretions like saliva, tears, and breast milk, IgA specialized in neutralizing pathogens, preventing toxins and microbes from adhering to epithelial cells, and maintaining a healthy balance with the body's natural microbiota.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Human IgA, Human IgA Heavy Chain |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.