Human IgA1 Recombinant monoclonal antibody

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• Human IgA1 Recombinant monoclonal antibody (87606-3-RR)

Human IgA1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 87606-3-RR

Clone No.252592A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

Human IgA, Human IgA Heavy Chain, IGHA1, IgA, IgA1

• 

  WB analysis of human milk using 87606-3-RR

  human milk were subjected to SDS PAGE followed by western blot with 87606-3-RR (Human IgA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 87606-3-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87606-3-RR (Human IgA1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human tonsillitis using 87606-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using Human IgA1 antibody (87606-3-RR, Clone: 252592A11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human milk
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87606-3-RR targets Human IgA1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg9190

  Product name: Recombinant human IgA1-IgK protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 10011193 of

  Sequence: \

• Full Name: immunoglobulin heavy constant alpha 1
• Observed Molecular Weight: 50-70 kDa
• Gene Symbol: Human IgA Heavy Chain
• Gene ID (NCBI): 3493
• RRID: AB_3745646
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human IgA1 is one of the two subclasses of Immunoglobulin A (IgA), the most abundantly produced antibody in the human body . While IgA is the predominant antibody class at mucosal surfaces (such as the gut, respiratory, and urogenital tracts), IgA1 is the dominant subclass in the blood (serum), comprising approximately 85-90% of total serum IgA .