Human IgG Polyclonal antibody

Human IgG Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 10284-1-AP

Synonyms

Human IgG



Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10284-1-AP targets Human IgG in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein
Full Name Human IgG
Calculated molecular weight 155 kDa
Observed molecular weight 25 kDa, 50 kDa
Gene symbol
Gene ID (NCBI)
RRIDAB_2877729
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This rabbit poly clonal antibody recognizes the human IgG.

Protocols

Product Specific Protocols
WB protocol for Human IgG antibody 10284-1-APDownload protocol
IHC protocol for Human IgG antibody 10284-1-APDownload protocol
IF protocol for Human IgG antibody 10284-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Sci Adv

Therapeutic strategies targeting uPAR potentiate anti-PD-1 efficacy in diffuse-type gastric cancer.

Authors - Long Qin

Int Immunopharmacol

LncRNA-H19 induces hepatic stellate cell activation via upregulating alcohol dehydrogenase III-mediated retinoic acid signals.

Authors - Zhi-Min Wang

Front Bioeng Biotechnol

Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes.

Authors - Rui-Rui Guo

Biosensors (Basel)

Compact Surface Plasmon Resonance IgG Sensor Based on H-Shaped Optical Fiber.

Authors - Yijian Huang
CoIP

Int J Oral Sci

FAM64A promotes HNSCC tumorigenesis by mediating transcriptional autoregulation of FOXM1.

Authors - Xinyuan Zhao
humanWB

Clin Exp Metastasis

Mass spectrometry-based proteomic capture of proteins bound to the MACC1 promoter in colon cancer.

Authors - Yahui Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Fatima (Verified Customer) (12-11-2018)

  • Applications: Flow Cytometry,