Rabbit Anti-Human IgG Polyclonal antibody

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• Rabbit Anti-Human IgG Polyclonal antibody (10284-1-AP)

Rabbit Anti-Human IgG Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 10284-1-AP

Publications(39)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF-P, IP, CoIP, ChIP, RIP, ELISA

Synonyms

Anti-Human IgG, Human IgG

• 

  WB analysis of human plasma using 10284-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 10284-1-AP (Human IgG antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of human plasma using 10284-1-AP

  IP result of Rabbit Anti-Human IgG (IP:10284-1-AP, 4ug; Detection:10284-1-AP 1:10000) with human plasma lysate 2600 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 10284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10284-1-AP (Rabbit Anti-Human IgG antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 10284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10284-1-AP (Rabbit Anti-Human IgG antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human tonsillitis using 10284-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Human IgG antibody (10284-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human plasma tissue
• Positive IP detected in: human plasma
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10284-1-AP targets Rabbit Anti-Human IgG in WB, IHC, IF-P, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: Rabbit Anti-Human IgG
• Calculated Molecular Weight: 155 kDa
• Observed Molecular Weight: 25 kDa, 50 kDa
• RRID: AB_2877729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This rabbit poly clonal antibody recognizes the human IgG.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	ChIP	Cell Metab; Oncogenic fatty acid oxidation senses circadian disruption in sleep-deficiency-enhanced tumorigenesis; Authors - Fei Peng
		Cancer Res; Breast Cancer Stem Cells Secrete MIF to Mediate Tumor Metabolic Reprogramming that Drives Immune Evasion; Authors - Linlin Yan
human	ChIP	Drug Resist Updat; Endothelial SMAD1-MCAM axis facilitates sunitinib resistance and progression of clear cell renal cell carcinoma via LAMB1-ITGB1 signaling.; Authors - Hanzhong Zhang
mouse	WB	Nat Commun; Sterically chained amino acid-rich water-soluble carbon quantum dots as a robust tumor-targeted drug delivery platform; Authors - Wenjing Xie
	CoIP	Int J Oral Sci; FAM64A promotes HNSCC tumorigenesis by mediating transcriptional autoregulation of FOXM1.; Authors - Xinyuan Zhao
	ChIP	J Exp Clin Cancer Res; Histone acetyltransferase CSRP2BP promotes the epithelial-mesenchymal transition and metastasis of cervical cancer cells by activating N-cadherin; Authors - Xiaohui Yang

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Fatima (Verified Customer) (12-11-2018)

• Applications: Flow Cytometry,