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Tested Applications
| Positive WB detected in | human plasma |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67761-1-Ig targets Human IgG Kappa chain in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | Human IgG Kappa chain |
| Calculated Molecular Weight | 155 kDa |
| Observed Molecular Weight | ~25 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2918529 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
This antibody detects the Kappa chain of human IgG,
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Human IgG Kappa chain antibody 67761-1-Ig | Download protocol |
| WB protocol for Human IgG Kappa chain antibody 67761-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
