Speedy™ Human/Cynomolgus Monkey IgG One-Step ELISA Kit
Cat no : SE50326
Synonyms
Immunoglobulin G
Validation Data Gallery
Product Information
| Product name | Speedy™ Human/Cynomolgus Monkey IgG One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum, Human plasma, Cynomolgus monkey serum, Cynomolgus monkey plasma, Urine, Saliva, Human milk |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human/Cynomolgus Monkey |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3501 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 107% | 83%-124% |
| Urine | 104% | 96%-116% |
| Saliva | 98% | 93%-105% |
| Human milk | 100% | 96%-109% |
| Cynomolgus monkey serum | 103% | 101%-105% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.43 | 0.20 | 3.68 |
| 2 | 8 | 1.16 | 0.04 | 3.45 |
| 3 | 8 | 0.62 | 0.05 | 8.06 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.81 | 0.58 | 9.98 |
| 2 | 16 | 1.21 | 0.08 | 6.61 |
| 3 | 16 | 0.63 | 0.05 | 7.94 |
Background Information
Immunoglobulin G (IgG) is the most abundant antibody isotype in human serum. It is a monomeric glycoprotein composed of two identical heavy chains and two identical light chains, with a molecular weight of about 150 kDa. IgG plays a central role in adaptive immunity by mediating pathogen neutralization, opsonization, and complement activation. Its four subclasses (IgG1, IgG2, IgG3, and IgG4) exhibit distinct functional profiles and differential affinities for Fc receptors, enabling tailored immune responses. Notably, IgG is the only antibody class capable of crossing the placental barrier, providing passive immunity to newborns.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Immunoglobulin G |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.