IgG lambda chain Monoclonal antibody

Human IgG lambda chain Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D12B5

Cat no : 67762-1-Ig

Synonyms

IGLC, IGLC1

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Validation Data Gallery

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  WB analysis of human plasma using 67762-1-Ig

  80 nL human plasma were subjected to SDS PAGE followed by western blot with 67762-1-Ig (Human IgG lambda chain antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 67762-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67762-1-Ig (Human IgG lambda chain antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67762-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67762-1-Ig (Human IgG lambda chain antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67762-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67762-1-Ig (Human IgG lambda chain antibody) at dilution of 1:2500 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 67762-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Human IgG lambda chain antibody (67762-1-Ig, Clone: 1D12B5 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: human plasma
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:1250-1:5000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67762-1-Ig targets Human IgG lambda chain in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human IgG lambda chain fusion protein Ag14783
• Full Name: immunoglobulin lambda constant 1 (Mcg marker)
• Calculated Molecular Weight: 233 aa, 25 kDa
• Observed Molecular Weight: ~25 kDa
• GenBank Accession Number: BC007782
• Gene Symbol: IGLC1
• Gene ID (NCBI): 3537
• RRID: AB_2918530
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody detects Lambda chain of human IgG.