Human IgM Recombinant monoclonal antibody, PBS Only

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• Human IgM Recombinant monoclonal antibody, PBS Only (87380-1-PBS)

Human IgM Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 87380-1-PBS

Clone No.252244E5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

IGHM, IgM, MU, VH

• 

  WB analysis using 87380-1-RR (same clone as 87380-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87380-1-RR (Human IgM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87380-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 87380-1-RR (same clone as 87380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87380-1-RR (Human IgM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87380-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 87380-1-RR (same clone as 87380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87380-1-RR (Human IgM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87380-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity of 87380-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87380-1-RR against Human IgM were performed. The affinity constant is 0.827 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

87380-1-PBS targets Human IgM in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg6742

  Product name: Recombinant Human IgM protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 105-448 aa of BC020240

  Sequence: VIAELPPKVSVFVPPRDGFFGNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKESGPTTYKVTSTLTIKESDWLGQSMFTCRVDHRGLTFQQNASSMCVPDQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVTERTVDKSTEGEVSADEEGFENLW

• Full Name: immunoglobulin heavy constant mu
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC020240
• Gene Symbol: IGHM
• Gene ID (NCBI): 3507
• RRID: AB_3745499
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

This antibody detects the heavy chain of human IgM (~75 kDa).