Human KDM3A/JMJD1A ELISA Kit
Cat no : KE00397
Synonyms
KDM3A,KDM3A,JMJD1A,[histone H3]-dimethyl-L-lysine(
Validation Data Gallery
Product Information
KE00397 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human KDM3A/JMJD1A ELISA kit is to be used to detect and quantify protein levels of endogenous human KDM3A/JMJD1A. The assay recognizes human KDM3A/JMJD1A. An antibody specific for human KDM3A/JMJD1A has been pre-coated onto the microwells. The human KDM3A/JMJD1A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human KDM3A/JMJD1A is added to detect the captured human KDM3A/JMJD1A protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human KDM3A/JMJD1A ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.07 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 55818 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 101% | 100%-104% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 5.22 | 0.09 | 1.78 |
| 2 | 20 | 1.27 | 0.02 | 1.89 |
| 3 | 20 | 0.33 | 0.01 | 3.49 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 5.40 | 0.20 | 3.73 |
| 2 | 24 | 1.37 | 0.05 | 3.98 |
| 3 | 24 | 0.39 | 0.03 | 7.96 |
Background Information
Human KDM3 is also known as JMJD1 (Jumonji domain-containing 1) and JHDM2 (Jumonji C domain-containing histone demethylase 2). The KDM3 subfamily encompasses four members: KDM3A, KDM3B, KDM3C and KDM3D. Among them, KDM3A is especially known to promote cancer cell proliferation, survival, and migration as well as tumor metastasis; it is associated with poor prognosis in various cancer types. KDM3A is suggested to aid lung adenocarcinoma cells in evading the immune system of patients by upregulating forkhead box P3 (Foxp3) expression in regulatory T cells (Tregs).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | KDM3A,KDM3A,JMJD1A,[histone H3]-dimethyl-L-lysine( |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.