Human KIR2DL4/CD158d ELISA Kit
Cat no : KE00912
Synonyms
KIR2DL4, CD158 antigen-like family member D, CD158D, G9P, Killer cell immunoglobulin-like receptor 2DL4
Validation Data Gallery
Product Information
KE00912 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human KIR2DL4/CD158d ELISA kit is to be used to detect and quantify protein levels of endogenous human KIR2DL4/CD158d. The assay recognizes human KIR2DL4/CD158d. An antibody specific for human KIR2DL4/CD158d has been pre-coated onto the microwells. The human KIR2DL4/CD158d protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human KIR2DL4/CD158d is added to detect the captured human KIR2DL4/CD158d protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human KIR2DL4/CD158d ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3805 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 93% | 90%-98% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.28 | 0.15 | 2.75 |
| 2 | 8 | 1.29 | 0.03 | 2.40 |
| 3 | 8 | 0.63 | 0.03 | 4.00 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.04 | 0.30 | 5.97 |
| 2 | 16 | 1.22 | 0.08 | 6.65 |
| 3 | 16 | 0.63 | 0.02 | 3.17 |
Background Information
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells that regulate their responsiveness to signals received from their environment. Killer cell immunoglobulin-like receptor 2DL4 (KIR2DL4, also known as CD158D and KIR103AS) is an unusual member of the KIRs family, which activates the cytotoxicity of NK cells. KIR2DL4 has two immunoglobulin domains and a single immunoreceptor tyrosine-based inhibition motif (ITIM) in the cytoplasmic tail with weak inhibitory potential.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | KIR2DL4, CD158 antigen-like family member D, CD158D, G9P, Killer cell immunoglobulin-like receptor 2DL4 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.