Human KLF5 ELISA Kit
Cat no : KE00403
Synonyms
KLF5,Basic transcription element-binding protein 2
Validation Data Gallery
Product Information
KE00403 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human KLF5 ELISA kit is to be used to detect and quantify protein levels of endogenous human KLF5. The assay recognizes human KLF5. An antibody specific for human KLF5 has been pre-coated onto the microwells. The human KLF5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human KLF5 is added to detect the captured human KLF5 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human KLF5 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 688 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 103% | 99%-105% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4.77 | 0.43 | 9.09 |
| 2 | 20 | 1.11 | 0.07 | 6.70 |
| 3 | 20 | 0.32 | 0.03 | 8.67 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 4.74 | 0.30 | 6.30 |
| 2 | 24 | 2.12 | 0.19 | 8.87 |
| 3 | 24 | 0.39 | 0.03 | 7.14 |
Background Information
Krueppel-like factor 5 (KLF5, also known as BTEB2, CKLF, and IKLF) is a zinc finger transcription factor that plays a significant role in various cellular processes, including proliferation, apoptosis, autophagy, and migration. Due to its role as a transcription factor, it is predominantly found in the nucleus. KLF5 is frequently abnormally expressed in human cancers, including squamous carcinoma, gastrointestinal cancer, and colorectal cancer. Studies of KLF5 are associated with prognostic biomarkers, therapeutic targets, and potential cancer drugs.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | KLF5,Basic transcription element-binding protein 2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.