Human KLK3/PSA ELISA Kit
Cat no : KE00708
Synonyms
KLK3, APS, EC:3.4.21.77, Gamma-seminoprotein, Kallikrein 3
Validation Data Gallery
Product Information
KE00708 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human KLK3/PSA ELISA kit is to be used to detect and quantify protein levels of endogenous human KLK3/PSA. The assay recognizes human KLK3/PSA. An antibody specific for human KLK3/PSA has been pre-coated onto the microwells. The human KLK3/PSA protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human KLK3/PSA is added to detect the captured human KLK3/PSA protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human KLK3/PSA ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 4.2 pg/mL |
| Range | 9.4-600 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 354 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 77% | 75%-79% |
| Cell culture supernatant | 106% | 101%-113% |
| Urine | 105% | 101%-111% |
| Cell lysate | 105% | 102%-109% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 309.5 | 12.3 | 4.0 |
| 2 | 8 | 74.4 | 1.4 | 1.9 |
| 3 | 8 | 36.9 | 2.5 | 6.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 315.7 | 12.1 | 3.8 |
| 2 | 16 | 75.1 | 1.5 | 1.9 |
| 3 | 16 | 37.8 | 2.8 | 7.4 |
Background Information
KLK3 also known as Prostate-Specific Antigen (PSA), is a 33 kDa glycoprotein primarily synthesized by the epithelial cells of the prostate gland. Its main physiological function is to cleave seminogelins I and II in semen, leading to the liquefaction of the seminal coagulum and the release of motile sperm. It is under tight regulation by androgens via the androgen receptor (AR). Serum PSA levels are elevated in prostate cancer due to disruption of the glandular architecture, allowing more PSA to enter the bloodstream. It is used alongside digital rectal exam (DRE) for early detection. Although termed "prostate-specific," PSA is not exclusively produced by the prostate. Very low levels can be produced in periurethral glands, breast tissue, and some other tissues. Its expression is highly abundant in prostatic tissue and seminal fluid.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | KLK3, APS, EC:3.4.21.77, Gamma-seminoprotein, Kallikrein 3 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.