Human KLKB1 ELISA Kit
Cat no : KE00690
Synonyms
Kininogenin, KLK3, KLKB 1, Plasma kallikrein, Plasma kallikrein B1
Validation Data Gallery
Product Information
KE00690 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human KLKB1 ELISA kit is to be used to detect and quantify protein levels of endogenous human KLKB1. The assay recognizes human KLKB1. An antibody specific for human KLKB1 has been pre-coated onto the microwells. The human KLKB1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human KLKB1 is added to detect the captured human KLKB1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human KLKB1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 1.6 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3818 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 89% | 80%-105% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,407.3 | 103.6 | 4.3 |
| 2 | 8 | 664.6 | 28.3 | 4.3 |
| 3 | 8 | 360.2 | 4.1 | 1.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,357.3 | 106.1 | 4.5 |
| 2 | 16 | 640.8 | 42.1 | 6.6 |
| 3 | 16 | 342.0 | 19.7 | 5.8 |
Background Information
Plasma kallikrein (KLKB1) is a serine protease that plays a crucial role in activating blood coagulation, fibrinolysis, kinin generation, and inflammation. It is synthesized in the liver and circulates in the plasma as a non-covalent complex with high molecular weight kininogen. KLKB1 can directly cleave the central complement component C3 to yield active components C3b and C3a, enhancing complement activation on cell surfaces. Additionally, the soluble form of KLKB1 is crucial for its role in the contact system, where it contributes to bradykinin generation and inflammation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Kininogenin, KLK3, KLKB 1, Plasma kallikrein, Plasma kallikrein B1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.