Human Kindlin 2 ELISA Kit
Cat no : KE00565
Synonyms
FERMT2, FERMT 2, KIND2, kindlin2, Kindlin-2
Validation Data Gallery
Product Information
KE00565 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Kindlin 2 ELISA kit is to be used to detect and quantify protein levels of endogenous human Kindlin 2. The assay recognizes human Kindlin 2. An antibody specific for human Kindlin 2 has been pre-coated onto the microwells. The human Kindlin 2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Kindlin 2 is added to detect the captured human Kindlin 2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Kindlin 2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.469-30 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10979 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 82% | 71%-95% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 15.07 | 0.27 | 1.81 |
| 2 | 8 | 3.83 | 0.12 | 3.06 |
| 3 | 8 | 1.98 | 0.05 | 2.74 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 14.46 | 1.07 | 7.41 |
| 2 | 16 | 3.70 | 0.23 | 6.12 |
| 3 | 16 | 1.87 | 0.14 | 7.49 |
Background Information
Kindlin 2, also named KIND2, MIG2, PLEKHC1, MIG-2 and FERMT2, belongs to the kindlin family. Three Kindlin 2 isoforms participate in the connection between extracellular matrix (ECM) adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Kindlin 2 recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites. Variable expression in human cancers and mesenchymal cancer invasion are now linked with FERMT2, which may play an important role in carcinormas development.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FERMT2, FERMT 2, KIND2, kindlin2, Kindlin-2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.