Human Kininogen 1 ELISA Kit
Cat no : KE00608
Synonyms
KNG1, Alpha-2-thiol proteinase inhibitor, BDK, Bradykinin, Fitzgerald factor
Validation Data Gallery
Product Information
KE00608 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Kininogen 1 ELISA kit is to be used to detect and quantify protein levels of endogenous human Kininogen 1. The assay recognizes human Kininogen 1. An antibody specific for human Kininogen 1 has been pre-coated onto the microwells. The human Kininogen 1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Kininogen 1 is added to detect the captured human Kininogen 1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Kininogen 1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Saliva |
| Assay type | Sandwich |
| Sensitivity | 0.06 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3827 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 94% | 92%-99% |
| Saliva | 107% | 93%-122% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.23 | 0.51 | 9.67 |
| 2 | 8 | 1.15 | 0.10 | 8.73 |
| 3 | 8 | 0.66 | 0.05 | 7.89 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.09 | 0.45 | 8.87 |
| 2 | 16 | 1.15 | 0.11 | 9.67 |
| 3 | 16 | 0.62 | 0.05 | 8.78 |
Background Information
Kininogen 1 (also known as high molecular weight kininogen, HMWK, or Fitzgerald factor) is a multidomain glycoprotein found in the blood of most vertebrate. Kininogen 1 is a key component of the kallikrein-kinin system (KKS), which, together with coagulation factor XI, forms the contact activation system of blood coagulation (CAS), also known as the intrinsic pathway of coagulation. This system contributes to thrombin generation primarily under pathologic conditions and can operate independently from the tissue factor (TF) extrinsic pathway. Kininogen 1 and its fragments have been found to have various biological functions. For example, kininogen 1 can regulate leukocyte recruitment, and its domain 5 (kininostatin) can down-regulate endothelial cell proliferation and migration, inhibiting angiogenesis. Additionally, kininogen 1 has been identified as a potential biomarker for early detection of advanced colorectal adenoma and colorectal cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | KNG1, Alpha-2-thiol proteinase inhibitor, BDK, Bradykinin, Fitzgerald factor |