Human LAIR1 ELISA Kit
Cat no : KE00785
Synonyms
CD305, hLAIR1, LAIR 1, LAIR-1, Leukocyte-associated immunoglobulin-like receptor 1
Validation Data Gallery
Product Information
KE00785 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human LAIR1 ELISA kit is to be used to detect and quantify protein levels of endogenous human LAIR1. The assay recognizes human LAIR1. An antibody specific for human LAIR1 has been pre-coated onto the microwells. The human LAIR1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human LAIR1 is added to detect the captured human LAIR1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human LAIR1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine |
| Assay type | Sandwich |
| Sensitivity | 2.5 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3903 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 93% | 88%-101% |
| Urine | 82% | 77%-90% |
| Cell culture supernatant | 97% | 94%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 560.6 | 24.8 | 4.4 |
| 2 | 8 | 136.7 | 4.0 | 3.0 |
| 3 | 8 | 44.4 | 1.3 | 2.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 551.6 | 23.5 | 4.3 |
| 2 | 16 | 136.3 | 3.3 | 2.4 |
| 3 | 16 | 42.2 | 2.7 | 6.3 |
Background Information
Leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) is an inhibitory receptor expressed on various immune cells, including T cells, B cells, monocytes, and dendritic cells. It plays a crucial role in immune regulation by recognizing collagen and other extracellular matrix components, leading to the inhibition of immune cell activation and cytokine production. LAIR1 is involved in the suppression of inflammatory responses and the maintenance of immune homeostasis. In cancer, LAIR1 can inhibit antitumor immune responses by downregulating the activity of cytotoxic T cells and promoting the function of regulatory T cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD305, hLAIR1, LAIR 1, LAIR-1, Leukocyte-associated immunoglobulin-like receptor 1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.